Entry vectors with fluorescent protein fusions

pENTR4-GFP-C1 (w392-1)

High-resolution pdf: pENTR4-GFP-C1 (w392-1).pdf

Sequence file: pENTR4-GFP-C1 (w392-1).seq

Sequence verification file: w392-1ENTRforw.ab1

Addgene #:17396

Recommended E.coli strain for propagation: TOP10F'.

Selection: Kanamycin.

Compatible Destination vectors: pLenti CMV, CMV/TO, PGK series, pQCXI CMV/TO series, pQCXP CMV/TO

 

pENTR4-GFP-C1X (pENTRGFP2)*

High-resolution pdf: pENTR4-GFP-C1X (pENTRGFP2).pdf

Sequence file: pENTR4-GFP-C1X (pENTRGFP2).seq

Addgene #:22450

Recommended E.coli strain for propagation: TOP10F'.

Selection: Kanamycin.

Compatible Destination vectors: pLenti CMV, CMV/TO, PGK series, pQCXI CMV/TO series, pQCXP CMV/TO.

The pENTR4-GFP-C1X plasmid contains a termination codon but not the pENTR4-GFP-C1 plasmid.

*Vector made by the Nathan Lawson lab, University of Massachusetts Medical School (Lawson lab web site).

 

pENTR4-GFP-C3 (w393-1)

High-resolution pdf: pENTR4-GFP-C3 (w393-1).pdf

Sequence file: pENTR4-GFP-C3 (w393-1).seq

Sequence verification file: w393-1ENTRforw.ab1

Addgene #:17397

Recommended E.coli strain for propagation: TOP10F'.

Selection: Kanamycin.

Compatible Destination vectors: pLenti CMV, CMV/TO, PGK series, pQCXI CMV/TO series, pQCXP CMV/TO

 

pENTR1A-GFP-N2 (FR1)*

High-resolution pdf: pENTR1A-GFP-N2 (FR1).pdf

Sequence file: pENTR1A-GFP-N2 (FR1).seq

Sequence verification file: pFR1ENTRforw.ab1

Addgene #:19364

Recommended E.coli strain for propagation: TOP10F'.

Selection: Kanamycin.

Recommended Destination vectors: pLenti CMV, CMV/TO, PGK series, pQCXI CMV/TO series, pQCXP CMV/TO.

*Vector made by Francis Rodier, Lawrence Berkeley National Laboratory.

 

pENTR4-DsRedEx2-C1 (pENTRdsRedEx2)*

High-resolution pdf: pENTR4-DsRedEx2-C1 (pENTRDsRedEx2).pdf

Sequence file: pENTR4-DsRedEx2-C1 (pENTRDsRedEx2).seq

Addgene #:22451

Recommended E.coli strain for propagation: TOP10F'.

Selection: Kanamycin.

Recommended Destination vectors: pLenti CMV, CMV/TO, PGK series, pQCXI CMV/TO series, pQCXP CMV/TO.

DsRed-Express is a rapidly maturing variant of Discosoma sp. red fluorescent protein (DsRed) that contains nine amino acid substitutions which enhance its solubility, reduce its green emission, and accelerate its maturation. Although DsRed-Express most likely forms the same tetrameric structure as wild-type DsRed, DsRed-Express displays a reduced tendency to aggregate. DsRed-Express2 has higher solubility, and was designed to be better suited for cell and stem cell applications (taken fron Clontech's web site). Link here.

*Vector made by the Nathan Lawson lab, University of Massachusetts Medical School (Lawson lab web site).

 

pENTR-DsRed2-N1 (CMB1)*

High-resolution pdf: pENTR DsRed2 N1 (CMB1).pdf

Sequence file: pENTR DsRed2 N1 (CMB1).seq

Sequence verification file: pENTR-DsRedENTRforw.ab1

Addgene #:22523

Recommended E.coli strain for propagation: TOP10F'.

Selection: Kanamycin.

Recommended Destination vectors: pLenti CMV, CMV/TO, PGK series, pQCXI CMV/TO series, pQCXP CMV/TO.

*Vector made by Christian Beauséjour, Centre de recherche du CHU Ste-Justine. (Dr. Beauséjour's laboratory).

 


www.000webhost.com