Vectors expressing the Tetracycline repressor (TetR)

TetR in pENTR1A (559-1)

High-resolution pdf: TetR in pENTR1A (559-1).pdf

Sequence file: TetR in pENTR1A (559-1).seq

Sequence verification files:

Addgene #:22265

Recommended E.coli strain for propagation: TOP10F'.

Selection: Kanamycin.

Compatible Destination vectors: pLenti CMV, PGK series.

 

pLenti CMV rtTA3 Blast (w756-1)

High resolution pdf: pLenti CMV rtTA3 Blast (w756-1).pdf

Sequence file: pLenti CMV rtTA3 Blast (w756-1).seq

Addgene #: not released yet.

Recommended E.coli strain for propagation: Stbl3.

Selection: Ampicillin.

This vector can be used to make cell lines Tet-On Advanced. For more information about the Tet-On Advanced system, please visit Clontech's website (here). The rtTA3 element was a gift from Dr. Dominic Esposito.

 

pLenti CMV rtTA3 Hygro (w785-1)

High resolution pdf: pLenti CMV rtTA3 Hygro (w785-1).pdf

Sequence file: pLenti CMV rtTA3 Hygro (w785-1).seq

Addgene #: 26730

Recommended E.coli strain for propagation: Stbl3.

Selection: Ampicillin.

This vector can be used to make cell lines Tet-On Advanced. For more information about the Tet-On Advanced system, please visit Clontech's website (here). The rtTA3 element was a gift from Dr. Dominic Esposito.

 

pLenti CMV rtTA3G Blast (R980-M38-658)

Higj resolution pdf: pLenti CMV rtTA3G Blast (R980-M38-658).pdf

Sequence file: pLenti CMV rtTA3G Blast (R980-M38-658).seq

Addgene #:31797

Recommended E.coli strain for propagation: Stbl3.

Selection: Ampicillin.

NOTE: This vector can be used to make cell lines Tet-On 3G. For more information about the Tet-On 3G system, please visit Clontech's website (here).

This vector was made by Dominic Esposito (SAIC Frederick).

 

pLenti CMV TetR Blast (716-1)

High-resolution pdf: pLenti CMV TetR Blast (716-1).pdf

Sequence file: pLenti CMV TetR Blast (716-1).seq

Addgene #:17492

Recommended E.coli strain for propagation: Stbl3.

Selection: Ampicillin.