Tet-On Advanced Vectors
pLenti CMV rtTA3 Blast (w756-1).jpg)
High resolution pdf: pLenti CMV rtTA3 Blast (w756-1).pdf
Sequence file: pLenti CMV rtTA3 Blast (w756-1).seq
Addgene #: 26429
Recommended E.coli strain for propagation: Stbl3.
Selection: Ampicillin.
This vector can be used to make cell lines Tet-On Advanced. For more information about the Tet-On Advanced system, please visit Clontech's website (here). The rtTA3 element was a gift from Dr. Dominic Esposito.
pLenti CMVtight BLAST DEST (w762-1)
.jpg)
High resolution pdf:pLenti CMVtight Blast DEST (w762-1).pdf
Sequence file: pLenti CMVtight Blast DEST (w762-1).seq
Addgene #: 26434
Recommended E.coli strain for propagation before the LR reaction: DB3.1 or ccDB survival.
Selection before the LR reaction: Ampicillin AND Chloramphinecol.
Recommended E.coli strain for propagation after the LR reaction: Stbl3.
Selection after the LR reaction: Ampicillin.
Compatible Entry vectors: pENTR noccDB, pENTR with tags, degrons, NLS.
The CMVtight element was a gift from Dr. Dominic Esposito.
NOTE: If the vector pLenti CMV rtTA3 Blast (w756-1) was used to make the Tet-On cell line, this vector can not be selected for because the cell line will be already resistant to blasticidin. The rtTA3 element should be expressed under a different drug selection such as hygromycin using the pLenti CMV rtTA3 Hygro (w785-1) vector.
pLenti CMVtight Hygro DEST (w769-1)
.jpg)
High resolution pdf: pLenti CMVtight Hygro DEST (w769-1).pdf
Sequence file: pLenti CMVtight Hygro DEST (w769-1).seq
Addgene #: 26433
Recommended E.coli strain for propagation before the LR reaction: DB3.1 or ccDB survival.
Selection before the LR reaction: Ampicillin AND Chloramphinecol.
Recommended E.coli strain for propagation after the LR reaction: Stbl3.
Selection after the LR reaction: Ampicillin.
Compatible Entry vectors: pENTR noccDB, pENTR with tags, degrons, NLS.
The CMVtight element was a gift from Dr. Dominic Esposito.
NOTE: Do not use this vector if the Tet-On Advanced cell line was made with the pLenti CMV rtTA3 Hygro (w785-1) vector because the cell line will already be resistant to hygromycin.
pLenti CMVtight Neo DEST (w770-1)
.jpg)
High resolution pdf: pLenti CMVtight Neo DEST (w770-1).pdf
Sequence file: pLenti CMVtight Neo DEST (w770-1).seq
Addgene #: 26432
Recommended E.coli strain for propagation before the LR reaction: DB3.1 or ccDB survival.
Selection before the LR reaction: Ampicillin AND Chloramphinecol.
Recommended E.coli strain for propagation after the LR reaction: Stbl3.
Selection after the LR reaction: Ampicillin.
Compatible Entry vectors: pENTR noccDB, pENTR with tags, degrons, NLS.
The CMVtight element was a gift from Dr. Dominic Esposito.
pLenti CMVtight Puro DEST (w768-1)
.jpg)
High resolution pdf: pLenti CMVtight Puro DEST (w768-1).pdf
Sequence file: pLenti CMVtight Puro DEST (w768-1).seq
Addgene #: 26430
Recommended E.coli strain for propagation before the LR reaction: DB3.1 or ccDB survival.
Selection before the LR reaction: Ampicillin AND Chloramphinecol.
Recommended E.coli strain for propagation after the LR reaction: Stbl3.
Selection after the LR reaction: Ampicillin.
Compatible Entry vectors: pENTR noccDB, pENTR with tags, degrons, NLS.
The CMVtight element was a gift from Dr. Dominic Esposito.