pLenti CMV TetR Blast (716-1)

High-resolution pdf: pLenti CMV TetR Blast (716-1).pdf

Sequence file: pLenti CMV TetR Blast (716-1).seq

Addgene #17492

Recommended E.coli strain for propagation Stbl3.

Selection: Ampicillin.

USE: To make cell line amenable to inducible expression of a cDNA/miRNA/shRNA i.e. make a T-REx cell line. The cDNA/miRNA/shRNA needs to be under the control of either the CMV/TO or H1/TO promoter to be inducible, and not in a backbone encoding the blasticidin resistance gene because the pLenti CMV TetR Blast already contains it.