pLenti X2 Series
These vectors have the Gateway Destination cassette inserted in the 3'LTR of the viral backbone. Upon integration in the host genome, the 3'LTR is duplicated over the 5'LTR hence duplicating the expression cassette. However there is a size limitation of ~1.5 kb for the insert.
pLenti X2 Blast DEST (w46-2)
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High-resolution pdf: pLenti X2 Blast DEST (w46-2).pdf
Sequence file: pLenti X2 Blast DEST (w46-2).seq
Addgene #17391
Recommended E.coli strain for propagation before the LR Reaction: DB3.1 or ccdB survival.
Selection before LR Reaction: Ampicillin and Chloramphinecol.
Recommended E.coli strain for propagation after the LR Reaction: Stbl3.
Selection after LR Reaction: Ampicillin.
pLenti X2 Hygro DEST (w17-1)
High-resolution pdf: pLenti X2 Hygro DEST (w17-1).pdf
Sequence file: pLenti X2 Hygro DEST (w17-1).seq
Addgene #17295
Recommended E.coli strain for propagation before the LR Reaction: DB3.1 or ccdB survival.
Selection before LR Reaction: Ampicillin and Chloramphinecol.
Recommended E.coli strain for propagation after the LR Reaction: Stbl3.
Selection after LR Reaction: Ampicillin.
pLenti X2 Neo DEST (w18-1)
High-resolution pdf: pLenti X2 Neo DEST (w18-1).pdf
Sequence file: pLenti X2 Neo DEST (w18-1).seq
Addgene #17390
Recommended E.coli strain for propagation before the LR Reaction: DB3.1 or ccdB survival.
Selection before LR Reaction: Ampicillin and Chloramphinecol.
Recommended E.coli strain for propagation after the LR Reaction: Stbl3.
Selection after LR Reaction: Ampicillin.
pLenti X2 Puro DEST (w16-1)
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High-resolution pdf: pLenti X2 Puro DEST (w16-1).pdf
Sequence file: pLenti X2 Puro DEST (w16-1).seq
Addgene #17296
Recommended E.coli strain for propagation before the LR Reaction: DB3.1 or ccdB survival.
Selection before LR Reaction: Ampicillin and Chloramphinecol.
Recommended E.coli strain for propagation after the LR Reaction: Stbl3.
Selection after LR Reaction: Ampicillin.
pLenti X2 Zeo DEST (749-3)
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High-resolution pdf: pLenti X2 Zeo DEST (749-3).pdf
Sequence file: pLenti X2 Zeo DEST (749-3).seq
Addgene #21562
Recommended E.coli strain for propagation before the LR Reaction: DB3.1 or ccdB survival.
Selection before LR Reaction: Zeocin (50 ug/ml) in low-salt LB and Chloramphinecol.
Recommended E.coli strain for propagation after the LR Reaction: Stbl3.
Selection after LR Reaction: Zeocin (50 ug/ml) in low-salt LB.