pLenti X1 Series
These vectors are promoter-less and can be used to express either a cDNA, shRNA or miRNA with its own promoter. The pEF-ENTR, pENTR/pTER, pENTR/pSUPER or pENTR/pSM2 Entry vectors can supply the promoter to express a cDNA (pEF-ENTR), an shRNA (pENTR/pTER or SUPER) or a miRNA (pENTR/pSM2 series). Alternatively, a promoter with a cDNA/shRNA/miRNA can be cloned into an Entry vector and recombined with the pLenti X1 series.
pLenti X1 Puro DEST (694-6)
High-resolution pdf: pLenti X1 Puro DEST (694-6).pdf
Sequence file: pLenti X1 Puro DEST (694-6).seq
Addgene #17297
Recommended E.coli strain for propagation before the LR Reaction: DB3.1 or ccdB survival.
Selection before LR Reaction: Ampicillin and Chloramphinecol.
Recommended E.coli strain for propagation after the LR Reaction: Stbl3.
Selection after LR Reaction: Ampicillin.
pLenti X1 Zeo DEST (668-1)
High-resolution pdf: pLenti X1 Zeo DEST (668-1).pdf
Sequence file: pLenti X1 Zeo DEST (668-1).seq
Addgene #17299
Recommended E.coli strain for propagation before the LR Reaction: DB3.1 or ccdB survival.
Selection before LR Reaction: Zeocin (50 ug/ml) in low-salt LB and Chloramphinecol.
Recommended E.coli strain for propagation after the LR Reaction: Stbl3.
Selection after LR Reaction: Zeocin (50 ug/ml) in low-salt LB.
pLenti X1 GFP-Zeo (720-2)
High-resolution pdf: pLenti X1 GFP-Zeo DEST (720-2).pdf
Sequence file: pLenti X1 GFP-Zeo DEST (720-2).seq
Addgene #19277
Recommended E.coli strain for propagation before the LR Reaction: DB3.1 or ccdB survival.
Selection before LR Reaction: Ampicillin and Chloramphinecol.
Recommended E.coli strain for propagation after the LR Reaction: Stbl3.
Selection after LR Reaction: Ampicillin.