pLenti CMV/TO Series

These vectors are compatible with T-REx cell lines (Invitrogen). In the absence of the tetracycline repressor (TetR), they will show constitutive expression. Cell lines can be made T-REx with the pLenti CMV TetR Blast (716-1) plasmid.

pLenti CMV/TO DEST (775-1)

High-resolution pdf: pLenti CMV/TO DEST (775-1).pdf

Sequence file: pLenti CMV/TO DEST (775-1).seq

Addgene #17432

Recommended E.coli strain for propagation before the LR Reaction: DB3.1 or ccdB survival.

Selection before LR Reaction: Ampicillin and Chloramphinecol.

Recommended E.coli strain for propagation after the LR Reaction: Stbl3.

Selection after LR Reaction: Ampicillin.

 

pLenti CMV/TO Hygro DEST (693-2)

High-resolution pdf: pLenti CMV/TO Hygro DEST (693-2).pdf

Sequence file: pLenti CMV/TO Hygro DEST (693-2).seq

Addgene #17291

Recommended E.coli strain for propagation before the LR Reaction: DB3.1 or ccdB survival.

Selection before LR Reaction: Ampicillin and Chloramphinecol.

Recommended E.coli strain for propagation after the LR Reaction: Stbl3.

Selection after LR Reaction: Ampicillin.

 

pLenti CMV/TO Neo DEST (685-3)

High-resolution pdf: pLenti CMV/TO Neo DEST (685-3).pdf

Sequence file: pLenti CMV/TO Neo DEST (685-3).seq

Addgene #17292

Recommended E.coli strain for propagation before the LR Reaction: DB3.1 or ccdB survival.

Selection before LR Reaction: Ampicillin and Chloramphinecol.

Recommended E.coli strain for propagation after the LR Reaction: Stbl3.

Selection after LR Reaction: Ampicillin.

 

pLenti CMV/TO Puro DEST (670-1)

High-resolution pdf: pLenti CMV/TO Puro DEST (670-1).pdf

Sequence file: pLenti CMV/TO Puro DEST (670-1).seq

Addgene #17293

Recommended E.coli strain for propagation before the LR Reaction: DB3.1 or ccdB survival.

Selection before LR Reaction: Ampicillin and Chloramphinecol.

Recommended E.coli strain for propagation after the LR Reaction: Stbl3.

Selection after LR Reaction: Ampicillin.

 

pLenti CMV/TO Zeo DEST (644-1)

High-resolution pdf: pLenti CMV/TO Zeo DEST (644-1).pdf

Sequence file: pLenti CMV/TO Zeo DEST (644-1).seq

Addgene #17294

Recommended E.coli strain for propagation before the LR Reaction: DB3.1 or ccdB survival.

Selection before LR Reaction: Zeocin (50 ug/ml) and Chloramphinecol in low-salt LB.

Recommended E.coli strain for propagation after the LR Reaction: Stbl3.

Selection after LR Reaction: Zeocin (50 ug/ml) in low-salt LB.

 

pLenti CMV/TO GFP-Zeo DEST (719-1)

High-resolution pdf: pLenti CMV/TO GFP-Zeo DEST (719-1).pdf

Sequence file: pLenti CMV/TO Puro DEST (719-1).seq

Addgene #17431

Recommended E.coli strain for propagation before the LR Reaction: DB3.1 or ccdB survival.

Selection before LR Reaction: Ampicillin and Chloramphinecol.

Recommended E.coli strain for propagation after the LR Reaction: Stbl3.

Selection after LR Reaction: Ampicillin.