pENTR4-SB1 (w789-3)

High resolution pdf:pENTR4-SB1 (w789-3).pdf

Sequence file: pENTR4-SB1 (789-3).seq

Sequence verification files: w789-3ENTRforw.ab1, w789-3ENTRrev.ab1

Addgene #: 26956

Recommended E.coli strain for propagation: TOP10F'

Selection: Kanamycin.

NOTE: The SB1 epitope was characterized in Schaeffer et al. 2010 (NAR Apr;38(6):e91) as part of the SnAvi tag. The SB1 hybridoma cell line can be ordered from the Developmental Studies Hybridoma bank (link here). The tag works well for Western blots and immunofluorescence in our hands.

Recommended Destination vectors: pLenti CMV, CMV/TO, CMVtight, CMVTRE3G, PGK series, pQCX CMV/TO series.