Entry vectors with fluorescent protein fusions
pENTR4-GFP-C1 (w392-1)
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High-resolution pdf: pENTR4-GFP-C1 (w392-1).pdf
Sequence file: pENTR4-GFP-C1 (w392-1).seq
Sequence verification file: w392-1ENTRforw.ab1
Addgene #:17396
Recommended E.coli strain for propagation: TOP10F'.
Selection: Kanamycin.
Compatible Destination vectors: pLenti CMV, CMV/TO, PGK series, pQCXI CMV/TO series, pQCXP CMV/TO
pENTR4-GFP-C1X (pENTRGFP2)*
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High-resolution pdf: pENTR4-GFP-C1X (pENTRGFP2).pdf
Sequence file: pENTR4-GFP-C1X (pENTRGFP2).seq
Addgene #:22450
Recommended E.coli strain for propagation: TOP10F'.
Selection: Kanamycin.
Compatible Destination vectors: pLenti CMV, CMV/TO, PGK series, pQCXI CMV/TO series, pQCXP CMV/TO.
The pENTR4-GFP-C1X plasmid contains a termination codon but not the pENTR4-GFP-C1 plasmid.
*Vector made by the Nathan Lawson lab, University of Massachusetts Medical School (Lawson lab web site).
pENTR4-GFP-C3 (w393-1)
High-resolution pdf: pENTR4-GFP-C3 (w393-1).pdf
Sequence file: pENTR4-GFP-C3 (w393-1).seq
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Sequence verification file: w393-1ENTRforw.ab1
Addgene #:17397
Recommended E.coli strain for propagation: TOP10F'.
Selection: Kanamycin.
Compatible Destination vectors: pLenti CMV, CMV/TO, PGK series, pQCXI CMV/TO series, pQCXP CMV/TO
pENTR1A-GFP-N2 (FR1)*
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High-resolution pdf: pENTR1A-GFP-N2 (FR1).pdf
Sequence file: pENTR1A-GFP-N2 (FR1).seq
Sequence verification file: pFR1ENTRforw.ab1
Addgene #:19364
Recommended E.coli strain for propagation: TOP10F'.
Selection: Kanamycin.
Recommended Destination vectors: pLenti CMV, CMV/TO, PGK series, pQCXI CMV/TO series, pQCXP CMV/TO.
*Vector made by Francis Rodier, Lawrence Berkeley National Laboratory.
pENTR4-DsRedEx2-C1 (pENTRdsRedEx2)*
 map and mcs.jpg)
High-resolution pdf: pENTR4-DsRedEx2-C1 (pENTRDsRedEx2).pdf
Sequence file: pENTR4-DsRedEx2-C1 (pENTRDsRedEx2).seq
Addgene #:22451
Recommended E.coli strain for propagation: TOP10F'.
Selection: Kanamycin.
Recommended Destination vectors: pLenti CMV, CMV/TO, PGK series, pQCXI CMV/TO series, pQCXP CMV/TO.
DsRed-Express is a rapidly maturing variant of Discosoma sp. red fluorescent protein (DsRed) that contains nine amino acid substitutions which enhance its solubility, reduce its green emission, and accelerate its maturation. Although DsRed-Express most likely forms the same tetrameric structure as wild-type DsRed, DsRed-Express displays a reduced tendency to aggregate. DsRed-Express2 has higher solubility, and was designed to be better suited for cell and stem cell applications (taken fron Clontech's web site). Link here.
*Vector made by the Nathan Lawson lab, University of Massachusetts Medical School (Lawson lab web site).
pENTR-DsRed2-N1 (CMB1)*
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High-resolution pdf: pENTR DsRed2 N1 (CMB1).pdf
Sequence file: pENTR DsRed2 N1 (CMB1).seq
Sequence verification file: pENTR-DsRedENTRforw.ab1
Addgene #:22523
Recommended E.coli strain for propagation: TOP10F'.
Selection: Kanamycin.
Recommended Destination vectors: pLenti CMV, CMV/TO, PGK series, pQCXI CMV/TO series, pQCXP CMV/TO.
*Vector made by Christian Beauséjour, Centre de recherche du CHU Ste-Justine. (Dr. Beauséjour's laboratory).