This web site is designed as a resource to use the viral vectors I designed to express and deplete proteins in mammalian cells. An article describing these vectors with some examples of their usefullness is published on the PLoS ONE web site:
Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD. A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells. http://dx.plos.org/10.1371/journal.pone.0006529. Click here for the pdf.
Here, you will find updated information about these vectors as well as new vectors that will be released after the publication of the article. All these vectors are available through Addgene (http://www.addgene.org). I also included the current detailed procotols I am using to clone small-hairpin RNAs (shRNAs) as well as microRNAs (miRNAs) and the sequence and map of each vector.
I also included vectors that are not fully characterized in the future vector section. These vectors are already made, but they have not been fully analyzed. Some other vectors have been designed, but they are not made yet.
Comments, suggestions, feedbacks are welcome and I would like this web page to be an interaction forum where ideas, tricks and experience with these vectors could be shared openly so that everyone could benefit and do more efficient research.
Eric Campeau, December 2009.